descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 1979 English Publisher:Elsevier BVJournal:Clinica Chimica Acta, volume 95, pages 419-423 (issn: 0009-8981,

Authors: S.Stefan Ahrenstedt; Jan I. Thorell;

doi: 10.1016/0009-8981(79)90191-8

pmid: 487582

The production of antibodies to vitamin B-12

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Abstract

The conjugation of vitamin B-12 to HSA by means of a combined ethylcarbodiimide N-hydroxysuccinimide conjugation method at pH 5.5 is described. It gave a ratio of 2.7 mol vitamin B-12/mol HSA. Five rabbits were immunized with this conjugate emulsified in Freund's complete adjuvant. After one booster injection high titre antisera (1 : 450 000--1 : 1 500 00) to vitamin B-12 were collected.

Related Organizations

Lund University
Sweden

Keywords

Chemical Phenomena, Immune Sera, Succinimides, Carbodiimides, Chemistry, Vitamin B 12, Antibody Formation, Animals, Immunization, Rabbits, Haptens, Serum Albumin

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