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Analytical Biochemistry
Article . 1979 . Peer-reviewed
License: Elsevier TDM
Data sources: Crossref
Analytical Biochemistry
Article . 1980
Data sources: Europe PubMed Central
Analytical Biochemistry
Article
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An improved rapid assay of glycogen synthase

descriptionPublicationkeyboard_double_arrow_right Article 01 Dec 1979Publisher:Elsevier BVJournal:Analytical Biochemistry, volume 100, pages 371-372 (issn: 0003-2697, Copyright policy )
Authors: Emma Villa-Moruzzi; Teresa Locci-Cubeddu; Ettore Bergamini;

doi: 10.1016/0003-2697(79)90243-4

pmid: 118679

An improved rapid assay of glycogen synthase

Abstract

Abstract As an alternative to rapid filtration washing the glycogen free of any unreacted UDP-[14C]-glucose by ascending chromatography (ethanol:water, 2:1) can be used. This technique also makes the filter paper assay of glycogen synthase much faster: The samples are ready for liquid scintillation counting in 30 min. Among the other advantages offered by this procedure, we should also mention that blanks are very low, large volumes of ethanol can be saved, and the unreacted UDP-[14C]glucose can be recovered by elution and recycled (it migrates with the front of the solvent).

Keywords

Uridine Diphosphate Glucose, Glycogen Synthase, Chromatography, Paper, Carbon Radioisotopes

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
8
Average
Average
Average
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