pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
Copyright policy )pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates.
- Academy of Sciences Library Czech Republic
- Czech Academy of Sciences Czech Republic
- University of Münster Germany
azo dyes, Science (General), protein grid, Computer applications to medicine. Medical informatics, R858-859.7, Azo dyes, pI, Comparative fluorescence gel electrophoresis, comparative fluorescence gel, hCoFGE, Gel electrophoresis, Q1-390, 2D-PAGE, electrophoresis, pI-Control, Protein grid, G-dyes, CoFGE, Data Article
azo dyes, Science (General), protein grid, Computer applications to medicine. Medical informatics, R858-859.7, Azo dyes, pI, Comparative fluorescence gel electrophoresis, comparative fluorescence gel, hCoFGE, Gel electrophoresis, Q1-390, 2D-PAGE, electrophoresis, pI-Control, Protein grid, G-dyes, CoFGE, Data Article
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