Sequence Analysis of Spiroplasma phoeniceum and Spiroplasma kunkelii Spiralin Genes and Comparison with Other Spiralin Genes

descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1997 France Publisher:Springer Science and Business Media LLCJournal:Current Microbiology, volume 35, pages 240-243 (issn: 0343-8651, eissn: 1432-0991,

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Authors: Foissac, Xavier; Bové, J.M.; Saillard, C.;

doi: 10.1007/s002849900246

pmid: 9290066

Sequence Analysis of Spiroplasma phoeniceum and Spiroplasma kunkelii Spiralin Genes and Comparison with Other Spiralin Genes

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Abstract

The spiralin genes from two phytopathogenic spiroplasmas, Spiroplasma phoeniceum and Spiroplasma kunkelii, were amplified by PCR, cloned, and sequenced. Comparison of the amino acid sequences of the five spiralins analyzed to date confirm that the spiralins have a general amphiphilic character and possess a conserved lipoprotein signal peptide. It also shows that a conserved central region and an amino acid repetition, including a VTKXE consensus sequence, are present in all spiralins analyzed.

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France

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Keywords

Base Sequence, Sequence Homology, Amino Acid, [SDV]Life Sciences [q-bio], Spiroplasma, Molecular Sequence Data, Protein Sorting Signals, Polymerase Chain Reaction, [SDV] Life Sciences [q-bio], Genes, Bacterial, Consensus Sequence, Amino Acid Sequence, Cloning, Molecular, Conserved Sequence, DNA Primers

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