A total of 132 Fusarium isolates belonging to 19 species sensu Nelson et al (1983) originating from Poland, Italy, and international cultures collections were examined for their ability to produce mycotoxin moniliformin.Moniliformin was produced by the following isolates:-F acuminatum Ell & Ev: 2 out of 2,130 - 2670mg/kg-F avenaceum (Fr) Sacc 18 out of 18,70 - 2670mg/kg-F anthophilum (A Braun) Wollenw. 1 out of 3, 200mg/kg-F dlamini Marasas et al: 2 out of 3,130 - 470mg/kg-F oxysporum Schlecht emend Snyd Hans: 4 out of 9,130 - 270 mg/kg-F proliferatum (Matsushima) Nirenberg: 3 out of 7,130 - 400 mg/kg-F solani (Mart) Appel & Wollenw: 1 out of 14,670 mg/kg-F subglutinans (Wollenw & Reinking) Nelson et al: 8 out of 20,70 - 1660 mg/kg-F tricinctum (Corda) Sacc: 2 out of 9,130 - 1330 mg/kgIn cultures ofF beomiforme Nelson, Toussoun & Burgess,F chlamydosporum Wollenw & Reinking,F compactum I Wollenw/ Gordon, F equiseti /Corda/Sacc,F poae I Peck / Wollenw,F moniliforme Sheldon,F napiforme Marasas, Nelson & Rabie,F nygamai Burgess & Timbold,F poly phialidicum Marasas et al,F sporotrichioides Sherb moniliformin was not detected.The highest amounts of moniliformin byF avenaceum using solid substrate were formed on rice and lower on oats kernels.