A micro pulsating heat pipe made of a thin clear Teflon tube of 1.6 mm ID was used to observe the pulsating flow inside a heat pipe under different gravity levels using parabolic flights. More vigorous pulsating flow was observed under microgravity, compared to the depressed movements under hypergravity. Two metallic micro pulsating heat pipes made of an aluminum plate with small internal channels were also tested to investigate the effect of gravity on their heat transfer characteristics. Reduced gravity experiments were performed aboard Falcon 20 aircraft flying parabolic trajectories. Under normal and hypergravity conditions, both the orientation of the pulsating heat pipe and locations of the heated and cooled sections affected the heat transfer performance. Under reduced gravity, however, the heat pipes showed better operating and heat transfer performance than that under normal and hypergravity. These experiments have for the first time confirmed that pulsating heat pipes are capable of operating under reduced gravity and thus are suitable for deployment in space applications such as satellites.