To induce mutation in tomatillo (Physalis ixocarpa Brot.) commonly grown for its fruit value, seeds were treated with three alkylating agents (dimethyl sulphate, diethyl sulphate and methyl ethane sulphonate) and gamma rays. Seven viable and five chlorophyll mutant types were screened both from M1 and M2 generations. The isolated mutant lines are described and evaluated with reference to their beneficial value.