A widely employed, comprehensive method exists for the determination of tuber total glycoalkaloids (TTGA). This method, which consists of a bisolvent extraction, preparation, and purification of aglycones and subsequent titration, was not acceptable for quantification of leaf total glycoalkaloids (LTGA). A modification of an existing extraction procedure was developed and tested. When the original bisolvent and the newly developed acetic acid extractions were contrasted percent recovery of a-solanine from leaf tissue was 19–27% and 91–97%, respectively. This new procedure was easily adapted for TTGA analysis and demonstrated improved recovery of α-solanine from tuber tissue when compared with the bisolvent extraction.