Analyses of blood chemical parameters were carried out in eight axis deer (Cervus axis), 12 fallow deer (Cervus dama), 16 red deer (Cervus elaphus hippelaphus), three sambar (Cervus unicolor), nine Pere David deer (Elaphurus davidianus), 20 European bison (Bison bonasus), seven nilgai (Boselaphus tragocamelus), eight mouflon (Ovis musimon), four white-bearded gnu (Connochaetes taurinus) and six barbary sheep (Ammotragus lervia). The following parameters were determined: glucose, urea, uric acid, cholesterol, triglycerides, creatinine, total bilirubin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, creatinine phosphokinase, lactate dehydrogenase, γ-glutamyl transpeptidase, total serum proteins, albumin, globulins, albumin/globulin ratio, sodium, potassium, chloride, calcium, magnesium, total phosphorus and serum osmolality. Some trends and statistically significant differences were observed between the species and the young and adult groups.