AbstractThe fatty acid composition ofSapindus mukorossi seed oil was determined by spectrophotometry, urea complexation, and gas liquid chromatography (GLC). The percentages of individual acids were found to be: palmitic, 4.0; stearic, 0.2; arachidic, 4.4; oleic 62.8; linoleic, 4.6; linolenic, 1.6; and eicosenoic, 22.4. Triglyceride composition was calculated from the fatty acid compositions of the native oil and of the monoglycerides produced from it by pancreatic lipase hydrolysis. The oil is composed of 0.1, 2.1, 22.0, and 75.8% trisaturated, monounsaturated disaturated, diunsaturated monosaturated, and triunsaturated glycerides, respectively. The special characteristic of theSapindus mukorossi seed oil is its content of 26.3 and 26.7% triolein and eicoseno‐di‐oleins, respectively.