AbstractEstolides were produced from meadowfoam oil fatty acids, oleic, linoleic, petroselinic, andcis‐5,cis‐13 docosadienoic acids. Estolide reaction mixtures were quantitated by Fourier transform infrared spectroscopy and compared to the area percentages determined by high‐performance liquid chromatography. The absorbance frequency of estolide carbonyl (1737 cm−1) is different than the lactone carbonyl (1790 cm−1) and the acid carbonyl (1712 cm−1). Estolide standards were obtained by wiped‐film molecular‐still distillations and column chromatography.