Near surface vertical distributions of Japanese anchovy eggs (isolated and pelagic) were studied at western Wakasa Bay, Japan. Samples were collected in horizontal tows with four plankton nets simultaneously operated at different four depths of 0, 0.5, 1.0, and 2.0 m. The egg concentration was found to decrease exponentially with depth. The egg concentration profile can be explained by considering the balance of the eggs' ascent and vertical diffusion. Taking further into account vertical difference in rising/sinking rates of the eggs, a clear accumulation of the eggs on a pycnocline, observed elsewhere, also can be explained. Vertical eddy diffusivity in the surface layer in a calm sea was 1–10 cm2 s−1, as estimated from the egg concentration profile and the velocities of the eggs in the vertical direction.