Production of waxy (amylose-free) wheats

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 1995 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 248, pages 253-259 (issn: 0026-8925, eissn: 1432-1874,

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Authors: T, Nakamura; M, Yamamori; H, Hirano; S, Hidaka; T, Nagamine;

doi: 10.1007/bf02191591

pmid: 7565586

Production of waxy (amylose-free) wheats

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Abstract

The Waxy (Wx) protein has been identified as granule-bound starch synthase (GBSS; EC 24.1.21), which is involved in amylose synthesis in plants. Although common wheat (Triticum aestivum L.) has three Wx proteins, "partial waxy mutants" lacking one or two of the three proteins have been found. Using such partial waxy mutants, tetra- and hexaploid waxy mutants with endosperms that are stained red-brown by iodine were produced. Both mutants showed loss of Wx protein and amylose. This is the first demonstration of genetic modification of wheat starch.

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Keywords

Polyploidy, Starch Synthase, Mutation, Electrophoresis, Polyacrylamide Gel, Triticum, Plant Proteins

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