Development of gene transfer methods forRubrivivax gelatinosus S1: construction, characterization and complementation of apuf operon deletion strain
Copyright policy )Development of gene transfer methods forRubrivivax gelatinosus S1: construction, characterization and complementation of apuf operon deletion strain
Gene transfer systems were developed in Rubrivivax (Rx.) gelatinosus S1. First, a system for conjugative transfer of mobilizable plasmids from Escherichia coli to Rx. gelatinosus S1 was established. Secondly, optimal conditions for the transformation of Rx. gelatinosus S1 by electroporation were determined. A delta puf strain was constructed. Complementation with the puf operon from a wild-type strain cloned in a replicative plasmid restored photosynthetic growth. Two insertion strains were also selected. All the strains constructed were green, due to a change in carotenoid content. Characterization of these strains provides genetic evidence for a "superoperon" organization in this bacterium.
Genetic Complementation Test, Photosynthetic Reaction Center Complex Proteins, Gene Transfer Techniques, Light-Harvesting Protein Complexes, Gene Expression Regulation, Bacterial, Rhodospirillaceae, Electroporation, Phenotype, Bacterial Proteins, DNA Transposable Elements, Escherichia coli, Transformation, Bacterial, Gene Deletion, Plasmids
Genetic Complementation Test, Photosynthetic Reaction Center Complex Proteins, Gene Transfer Techniques, Light-Harvesting Protein Complexes, Gene Expression Regulation, Bacterial, Rhodospirillaceae, Electroporation, Phenotype, Bacterial Proteins, DNA Transposable Elements, Escherichia coli, Transformation, Bacterial, Gene Deletion, Plasmids
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