Bioluminescence assay of calcium with a liquid scintillation counter

descriptionPublicationkeyboard_double_arrow_right Article 01 Aug 1983 English Publisher:Springer Science and Business Media LLCJournal:Experientia, volume 39, pages 928-930 (issn: 0014-4754, eissn: 1420-9071,

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Authors: C, Oveby; R, Kant; S, Batra;

doi: 10.1007/bf01990443

pmid: 6409664

Bioluminescence assay of calcium with a liquid scintillation counter

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Abstract

The luminescence produced by Ca aequorin reaction in dilute solutions decays exponentially over a relatively prolonged period of time. The concentration of total Ca and of free Ca in Ca-EGTA buffers could be determined by measuring the decay of luminescence in a liquid scintillation counter. The method is also suitable for determining total Ca concentration in small tissue samples.

Keywords

Uterus, Aequorin, Luminescent Measurements, Methods, Animals, Scintillation Counting, Calcium, Female, Rabbits, Egtazic Acid, Aorta

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