An improved spectrophotometric assay for histamine and diamine oxidase (DAO) activity
Copyright policy )An improved spectrophotometric assay for histamine and diamine oxidase (DAO) activity
Diamine oxidase or its substrates can be measured using a coupled reaction with peroxidase and the chromogen 3-methyl-2-benzothiazolone hydrazone (MBTH) with an appropriate acceptor such as 3-(dimethylamino)benzoic acid (DMAB). This method provides a more rapid and sensitive method of measuring histamine than current colorimetric assays; using this system, histamine was quantitated at concentrations of 10 to 400 mumol/l. The Km of DAO was determined to be 2.9 X 10(-5) M, a lower value than previously reported.
- University of Southern Mississippi United States
Thiazoles, Chromogenic Compounds, Spectrophotometry, Hydrazones, Amine Oxidase (Copper-Containing), Benzothiazoles, Hydrogen-Ion Concentration, Histamine
Thiazoles, Chromogenic Compounds, Spectrophotometry, Hydrazones, Amine Oxidase (Copper-Containing), Benzothiazoles, Hydrogen-Ion Concentration, Histamine
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