Hairy roots ? a short cut to transgenic root nodules

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1989 English Publisher:Springer Science and Business Media LLCJournal:Plant Cell Reports, volume 8, pages 12-15 (issn: 0721-7714, eissn: 1432-203X,

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Authors: Hansen, Jørgen; Jørgensen, Jan Elo; Stougaard, Jens; Marcker, Kjeld A.;

doi: 10.1007/bf00735768

pmid: 24232586

Hairy roots ? a short cut to transgenic root nodules

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Abstract

To facilitate molecular studies of symbiotic nitrogen fixation a procedure for rapid production of transgenic root nodules was established on the legumeLotus corniculatus (Bird'sfoot trefoil). Regeneration of transgenic plants is not required as transgenic nodules are formed onAgrobacterium rhizogenes incited roots inoculated withRhizobium. Easy identification of transformed roots is possible using a set ofA. rhizogenes acceptor strains carrying assayable marker genes such as chloramphenicol acetyltransferase (CAT), β-glucuronidase (GUS), or luciferase (LUC) under control of the cauliflower mosaic virus (CaMV) 35S promoter. Counterselection ofA. rhizogenes after infection of plants was improved using an auxotrophy marker.

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Aarhus University
Denmark

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