Influence of culture conditions on the production of milk-clotting enzyme from Rhizomucor

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1994 English Publisher:Springer Science and Business Media LLCJournal:World Journal of Microbiology & Biotechnology, volume 10, pages 527-530 (issn: 0959-3993, eissn: 1573-0972,

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Authors: S, Preetha; R, Boopathy;

doi: 10.1007/bf00367659

pmid: 24421126

Influence of culture conditions on the production of milk-clotting enzyme from Rhizomucor

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Abstract

The ratio of milk-clotting activity to proteolytic activity (MC/PA) was used as an index to determine the quality of milk-clotting enzyme. Solid-state fermentation on wheat bran for 5 days at room temperature gave optimal production for enzyme by Rhizomucor miehei and R. pusillus. A ratio of wheat bran to moisture of 1:0.6 (w/v) gave best results. Adding skim milk powder to the media of R. miehei did not improve the MC/PA ratio but 4% (w/w) of the powder did improve the ratio with R. pusillus. Co-cultivation of R. miehei with R. pusillus did not change the MC/PA ratio.

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