Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel
Copyright policy )Rapid detection of hypoxanthine-guanine phosphoribosyltransferase on cellogel
A simple, fast and direct staining method for the detection of hypoxanthineguanine phosphoribosyltransferase is described. It is based on the conversion of inosine monophosphate to hypoxanthine, which is then enzymatically oxidized. This oxidation is coupled to the reduction of a tetrazolium salt to blue formazan. The electrophoretic pattern of HGPRT in man-Chinese hamster hybrid cells shows a three-banded pattern, suggesting a dimeric structure for this enzyme.
- Delft University of Technology Netherlands
- Leiden University Netherlands
Staining, Electrophoresis, Guanine, Staining and Labeling, Animal, Hybrid cell, Hypoxanthine derivative, In vitro study, Hybrid Cells, In Vitro Techniques, In Vitro, Cricetinae, Hypoxanthines, Hamster, Hamsters, Purine nucleotide, Animals, Humans, Pentosyltransferases, Oxidation reduction reaction, Inosine Nucleotides, Oxidation-Reduction, Glycosyltransferase, Human
Staining, Electrophoresis, Guanine, Staining and Labeling, Animal, Hybrid cell, Hypoxanthine derivative, In vitro study, Hybrid Cells, In Vitro Techniques, In Vitro, Cricetinae, Hypoxanthines, Hamster, Hamsters, Purine nucleotide, Animals, Humans, Pentosyltransferases, Oxidation reduction reaction, Inosine Nucleotides, Oxidation-Reduction, Glycosyltransferase, Human
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