Vaginal penetration pressure (VPP) has been defined as the pressure recorded by introducing a truncated probe (diameter 2.9 cm) 5 cm into the vagina. A total of six women participated in this study, and pressure was recorded in the supine and kneeling positions. Measurements of VPP were, on the average, 21 mmHg higher in the superior position than in the supine. After clitoral self-stimulation the procedure was repeated and, in 7 of 8 measurements, a decrease of VPP (range 4–58 mmHg) was found. As efficient sexual stimulation and position of the woman are parameters influencing VPP, it is suggested that these be considered when taking a history in male patients with vaginal penetration problems. In certain cases, vaginal examination of the partner should be carried out.