Accurate navigation forms an essential part of all research at sea and the deep ocean imposes it’s own unique problems. This chapter discusses several of the techniques in current use on the research vessels of the Natural Environment Research Council (NERC), concentrating on those systems which provide global navigation facilities, as opposed to the more localised, coastal aids. Whilst most of the systems rely on surface propagation of radio waves, the use of acoustics and sea-bed mapping instruments constitute accurate alternatives for some sub-sea applications.