Plant regeneration from protoplasts of Capsicum annuum

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1988 English Publisher:Springer Science and Business Media LLCJournal:Plant Cell Reports, volume 7, pages 210-212 (issn: 0721-7714, eissn: 1432-203X,

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Authors: I, Díaz; R, Moreno; J B, Power;

doi: 10.1007/bf00269326

pmid: 24241602

Plant regeneration from protoplasts of Capsicum annuum

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Abstract

Leaf protoplasts were isolated from axenic shoot cultures of four varieties of Capsicum annuum (Americano, Dulce Italiano Florida Gynat and Nigrum) and a wild species C. chinense. Protoplasts of both species, cultured in KM8P medium and using agarose bead culture, entered division with the exception of the variety Nigrum. Cell colonies formed callus in agar-solified MS medium supplemented with zeatin and for C. annuum v. Dulce Italiano shoots were regenerated when protoplast-derived calli were transferred to MS medium with 6-BAP. Excised shoots were rooted on MS medium which lacked phytohormones.

Related Organizations

Spanish National Research Council
Spain
University of Nottingham
United Kingdom

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