Rapid plant regeneration of pea using thidiazuron

descriptionPublicationkeyboard_double_arrow_right Article 01 May 1996 English Publisher:Springer Science and Business Media LLCJournal:Plant Cell, Tissue and Organ Culture, volume 45, pages 165-168 (issn: 0167-6857, eissn: 1573-5044,

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Authors: Massimo H. M. Sanago; Vern I. Shattuck; Judith Strommer;

doi: 10.1007/bf00048761

Rapid plant regeneration of pea using thidiazuron

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Abstract

A simple and rapid pea regeneration procedure was developed. An average of up to 20 shoots formed from hypocotyl explants of cvs. Sugar Ann and Patriot cultured on Murashige and Skoog basal medium supplemented with 0.5 or 1.0 μM thidiazuron (N-phenyl-N′-1,2,3-thiadiazol-5′-ylurea). Hypocotyls of Puget and Sugar Daddy did not respond. Regenerated shoots rooted rapidly when cultured on Murashige and Skoog basal medium containing either 2.0 μM α-naphthaleneacetic acid or 1.0–2.0 μM indole-3-butyric acid. Seeds were harvested from regenerated plants after only 9–11 weeks.

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University of Guelph
Canada

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