A Giemsa-C-banded karyotype of a partial amphiploid, Triticum turgidum L. var. durum cv. ‘Nodak’ × Agropyron intermedium (Host) P.B., called MT-2, was analyzed. MT-2 is a winterhardy grasslike octoploid germplasm which survived 5 winters in Montana, and its seed weight is 3 times that of A. intermedium seed. The MT-2 C-banding karyotype shows 6 chromosome pairs each of the A and B wheat genomes with 3A and 4B missing. Chromosomes 1B and 2B are involved in a reciprocal homozygous translocation (T1BS·2BS, T1BL·2BL) which was also confirmed by a nucleolus-associated quadrivalent in an MT-2 × durum wheat backcross. In addition to the wheat chromosomes, MT-2 consistently shows 16 A. intermedium chromosome pairs which are designated from A to P. These chromosomes show C-banding patterns similar to those reported earlier in the literature. A large amount of C-banding polymorphism and structural rearrangements in A. intermedium itself presently make a definite chromosome assignment to the homeologous groups of the Triticeae difficult. The data presented are crucial for further directed manipulation of this germplasm aimed at producing valuable chromosome additions and substitutions in wheat.