Somatic embryos and adult plants were regenerated from mesophyll protoplasts of a clone of chicory ‘474’ (Cichorium intybus L. x Cichorium endivia L.). Embryos were obtained in three different ways: - plating of 7-day-old protoplast cultures on PM-3 solid induction medium with 2.5 μM 2-isopentenyladenine (2-iP) and 0.5 μM naphthaleneacetic acid (NAA); - transfer of 1–2 mm microcolonies into liquid M-17 induction medium with 2.5 μM (2-iP) and 0.1 μM (NAA); - transfer of well-established protoplast-derived calluses onto M-17.