The 5S-rRNA from the ungerminated and 48-h-germinated rice embryos differs from the wheat, rye and maize by two nucleotides. The 48-h-germinated embryos contain another species of 5S-rRNA which differs by 3 nucleotides from the ungerminated embryos, thereby showing the expression of two 5S-rRNA genes during germination. The 5S-rRNA genes are present in tandem repeats of a 0.3-kb sequence with some length heterogeneity in the rice genome. The 5S-rRNA gene that was sequenced is identical to that of wheat and maize, except for two nucleotides, C and T, which are interchanged at positions 107 and 117. The insert of continuous 5S-rRNA gene in pBR322 was transcribed in vitro much more efficiently than the discontinuous gene. There was no homology between the 184-bp spacer sequence of 5S-rRNA genes in rice and other systems except the presence of the oligo(T) transcription terminator sequence.