
A comparative study of the three gastropod species Nucella lapillus (L.), Ocenebra erinacea (L.) and Hinia (Nassarius) reticulata (L.) reveals that Nucella is the most TBT sensitive species while Hinia is the least sensitive. Of the two imposex indices VDSI and RPSI, good interspecies correlations were obtained only for VDSL The three species can be considered as complementary not only in terms of their ecology but also for their levels of sensitivity. Indeed, Nucella and Ocenebra are useful test species at TBT concentrations below 2 ng Sn 1-1 while Hinia is the more appropriate species at higher TBT levels. For the first time, two sterilised Hinia females are recorded. This sterilization does not seem to be due to proliferation of vas deferens tissue in the vaginal opening and further investigation is needed to find intermediary VDS stages between stage 4+ and sterilization. Studies using Nucella show that the use of narcotization in imposex analysis leads to an underestimation of RPSI compared with non-narcotization methods. Indeed, narcotization straightens the penis and increases its length when compared with non-narcotized animals, but this increase is proportionally higher in males than in females.
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