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Part of book or chapter of book . 1991 . Peer-reviewed

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Ribosomal RNA Sequencing

Name: Ribosomal RNA Sequencing
Creator: Michel Solignac
Keywords: 3. Good health

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Other literature type 01 Jan 1991Publisher:Springer Berlin Heidelberg

Authors: Michel Solignac;

doi: 10.1007/978-3-642-83962-7_24

Ribosomal RNA Sequencing

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Abstract

Ribosomal genes can be cloned or PCR amplified and then sequenced as other DNA sequences. However, the products of the genes, ribosomal RNAs, can be used as template for direct sequencing with the reverse transcriptase as polymerase following a protocol derived from the Sanger’s technics for DNA (dideoxynucleotide chain termination). Universal primers can be used to sequence the chosen region of the rRNA.

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	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
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