Detecting p53 Isoforms at Protein Level
Detecting p53 Isoforms at Protein Level
The human p53 protein isoforms are expressed in several cell lines and modulate p53 tumor suppressor -activity, mainly through modulation of gene expression (1-4). Thus, identifying the pattern of p53 isoforms expression in cell lines is a key step for future studies of the p53 network (5). At the moment, the detection of p53 protein isoforms is based on the use of a panel of antibodies allowing their identification by comparing their molecular weights and their detection pattern by different antibodies (6). Here, classical protocols supplemented with technical know-how are described to detect p53 protein isoforms at protein level by Western blotting and immunoprecipitation. Furthermore, a simple method to study the impact of p53 protein isoforms on p53 transcriptional activity through luciferase reporter gene assays is provided.
- Ninewells Hospital United Kingdom
- NHS Tayside United Kingdom
- University of Dundee United Kingdom
Transcriptional Activation, 570, Blotting, Western, 610, Gene Expression, Antibodies, Western blotting, Cell Line, Genes, Reporter, Animals, Humans, Immunoprecipitation, Protein Isoforms, Animal model, Protein detection, Luciferases, Zebrafish, Luciferase reporter gene assays, p53 tumor suppressor protein, p53 protein isoforms, [SDV] Life Sciences [q-bio], p53 protein isoforms Antibodies Western blotting Immunoprecipitation Luciferase reporter gene assays Protein detection p53 tumor suppressor protein Animal model, Models, Animal, Drosophila, Tumor Suppressor Protein p53
Transcriptional Activation, 570, Blotting, Western, 610, Gene Expression, Antibodies, Western blotting, Cell Line, Genes, Reporter, Animals, Humans, Immunoprecipitation, Protein Isoforms, Animal model, Protein detection, Luciferases, Zebrafish, Luciferase reporter gene assays, p53 tumor suppressor protein, p53 protein isoforms, [SDV] Life Sciences [q-bio], p53 protein isoforms Antibodies Western blotting Immunoprecipitation Luciferase reporter gene assays Protein detection p53 tumor suppressor protein Animal model, Models, Animal, Drosophila, Tumor Suppressor Protein p53
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