Osteoclasts and their precursors have traditionally been considered difficult cells to transfect using standard approaches. Here, we describe several methods for transfection of mature osteoclasts and their precursors using the Amaxa™ Nucleofector system, lentiviruses, and adenoviruses.

Related Organizations

University of Aberdeen
United Kingdom

Keywords

Cell Survival, Genetic Vectors, Lentivirus, Osteoclasts, Transfection, Adenoviridae, Cell Line, Mice, Electroporation, Transduction, Genetic, Animals, Humans, Cells, Cultured

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