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Genome Editing of Medaka

descriptionPublicationkeyboard_double_arrow_right Part of book or chapter of book , Article 01 Jan 2023Publisher:Springer US

Authors: Rie, Hara; Satoshi, Ansai; Masato, Kinoshita;

doi: 10.1007/978-1-0716-3016-7_25

pmid: 36773158

Genome Editing of Medaka

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Abstract

Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knockout strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knockin using a plasmid carrying a sequence that does not cause off-target effects in medaka. Additionally, knockin method without plasmid cloning is described.

Related Organizations

Tohoku University
Japan
Kyoto University
Japan

Keywords

Gene Editing, Gene Knockout Techniques, Oryzias, Animals, Zebrafish, Plasmids

Impact byBIP!

	citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	1
	popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.	Average
	influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).	Average
	impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.	Average