This review of embryogenesis from microspores in two types of wheat examines the influences of various procedures used to induce and produce embryoids from the male gametes. The various factors that influence the quality of the microspores are critical and include growth of donor plants, pretreatments and culture and regeneration media. There are also many genetic and genotype factors, with a major problem being production of albino plants. Genotype differences in response and gametic selection during culture may be overcome through improved response in culture such as has been achieved in barley. Chromosome doubling to obtain uniform doubled haploid lines is being continually improved through pretreatments to cause nuclear fusion and through anti-microtubule agents in the induction media. Finally, the interesting aspects of cell and tissue changes that occur during the induction and development of the embryoids are briefly described and illustrated.

Peer Reviewed