Abstract Twenty-nine megaspore species including six new taxa ( Bacutriletes otwayensis sp. nov., Erlansonisporites cerebrus sp. nov., Erlansonisporites decisum sp. nov., Hughesisporites coronatus sp. nov., Hughesisporites dettmanniae sp. nov., and Verrutriletes depressus sp. nov.) are documented from Aptian and Albian strata of the Gippsland and Otway basins, southeastern Australia. Together with six taxa known only from underlying Neocomian strata, these megaspores are used to establish four provisional biozones for the Lower Cretaceous that complement existing biostratigraphic schemes based on miospores and plant macrofossils. Megaspores are best represented in silty floodbasin facies and it is likely that the parent plants predominantly occupied moist understorey to fully aquatic habitats on the floodplain. Megaspores are sparsely represented in most other fluvial facies chiefly due to reworking of floodbasin sediments into higher energy channel and crevasse deposits. The relatively high diversity of lycophyte and fern megaspores contrasts with the scarcity of these plant groups in macrofossil assemblages. The megaspore record suggests that heterosporous cryptogams may have been significantly more prominent in the vegetation of this region than previously suggested. Several megaspores from southeastern Australia are closely comparable to forms from India and Argentina indicating broad similarities between Early Cretaceous heterosporous fern and lycophyte communities across Gondwana. These similarities also suggest that megaspores may be useful for inter-continental biostratigraphic correlation.