Ribosomal DNA spacer probes for yeast identification: Studies in the genus Metschnikowia
Copyright policy )Ribosomal DNA spacer probes for yeast identification: Studies in the genus Metschnikowia
AbstractTo test whether DNA probes derived from ribosomal DNA spacer sequences are suitable for rapid and species‐specific yeast identification, a pilot study was undertaken. A 7·7 kb entire ribosomal DNA unit of the type strain of Metschnikowia reukaufii was isolated, cloned and mapped. A 0·65 kb BamHI‐HpaI fragment containing nontranscribed spacer sequences was amplified and selected for testing as a 32P hybridization probe with total DNA from the type strains of M. reukaufii, M. pulcherrima, M. lunata, M. bicuspidata, M. australis, M. zobellii, M. krissii, five other strains identified as M. reukaufii and strains of Schizosaccharomyces pombe, Hansenula canadensis, Saccharomyces cerevisiae and Yarrowia lipolytica. The probe hybridized exclsively with DNA from the type strain and four other strains of M. reukaufii. DNA from one strain labelled M. reukaufii did not hybridize with the probe. Subsequent % G+C comparison and DNA–DNA reassociation with the type strain revealed that the non‐hybridizing strain does not belong to the species M. reukaufii.
Genetic Markers, Transcription, Genetic, Restriction Mapping, Nucleic Acid Hybridization, DNA, Ribosomal, Blotting, Southern, Species Specificity, Sequence Homology, Nucleic Acid, Saccharomycetales, Cloning, Molecular, DNA Probes, DNA, Fungal, Plasmids
Genetic Markers, Transcription, Genetic, Restriction Mapping, Nucleic Acid Hybridization, DNA, Ribosomal, Blotting, Southern, Species Specificity, Sequence Homology, Nucleic Acid, Saccharomycetales, Cloning, Molecular, DNA Probes, DNA, Fungal, Plasmids
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