QDs’ adsorption on multi‐surface is one of the key points to achieve on‐chip nano‐source. By click chemistry between surface functionalization layer and QDs, QDs were adsorbed on the target surface. This method could achieve small size, stable performance and high efficiency at the same time. Two kinds of surface functionalization material, with similar structure, were used to compare the QDs adsorption ability. DCOOH, the material with click chemistry possibility, exhibit 3‐time stronger adsorption ability comparing the DNO 2 .