The plants Rutaceae family are known to have contributed a lot toward food and medicine. The most important metabolites of the family are flavonoids. A systematic review was conducted to collect chemical and pharmacological information of flavonoids isolated from family Rutaceae till 2018. A plethora of flavonoids have been isolated and studied systematically for various bioactivities, including anticancer, antibacterial, antiviral, analgesic, antioxidant, antidiabetic, antiinflammatory, in bronchitis, ulcers, and so on. The important groups of flavonoids isolated are naringin, poncirin, rhoifolin, marmesin, hesperidin, tangeretin, nobiletin, glychalcone, glyflavanone, lemairone, acacetin 3,6‐di‐C‐glucoside, vicenin‐2, lucenin‐2 4′‐methyl ether, narirutin 4′‐O‐glucoside, apigenin 8‐C‐neohesperidoside, phloretin 3′,5′‐di‐C‐glucoside, rutin, rhamnetin, dihydrokaempferol, dihydrokaempferol 3‐O‐rhamnoside (engeletin) and kaempferol, excavaside A and B, myricetin 3‐O‐β‐D‐rutinoside, myricetin 3,3′‐di‐α‐l‐rhamnopyranoside, myricetin 3′‐α‐l‐rhamnopyranoside, and others. The flavonoids isolated from the citrus family need to be considered from a nutraceutical, therapeutic, and pharmaceutical point of view for future medicine.