A quantitative assay for Amaranthus palmeri identification

descriptionPublicationkeyboard_double_arrow_right Article 07 Aug 2017 English Publisher:WileyJournal:Pest Management Science, volume 73, pages 2,221-2,224 (issn: 1526-498X, eissn: 1526-4998,

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Authors: Brent P Murphy; Diane E Plewa; Elizabeth Phillippi; Suzanne M Bissonnette; Patrick J Tranel;

doi: 10.1002/ps.4632

pmid: 28580655

A quantitative assay for Amaranthus palmeri identification

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Abstract

AbstractBACKGROUNDAmaranthus palmeri recently has been brought into the Midwestern USA as a contaminant in Conservation Reserve Program seeding mixes. Rapid species screening is required to mitigate the risk of continued species movement.RESULTSMarkers were developed for A. palmeri‐specific nucleotide polymorphisms in the internal transcribed spacer of the ribosomal coding region. A quantitative polymerase chain reaction (qPCR) assay successfully identified A. palmeri from single‐plant samples, simulated mixed‐plant samples and seed mixtures.CONCLUSIONA qPCR assay for distinguishing A. palmeri from 12 other Amaranthus spp. was developed and validated. The assay can consistently detect a single A. palmeri seed when present in a pool of 100 total Amaranthus spp. seeds. © 2017 Society of Chemical Industry

Related Organizations

University of Illinois at Urbana Champaign
United States
University of Illinois System
United States

Keywords

Genetic Markers, Amaranthus, Polymorphism, Genetic, DNA, Plant, DNA, Ribosomal Spacer, Seeds, Polymerase Chain Reaction

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