A ubiquitin and ubiquitin‐like protein spectral library

descriptionPublicationkeyboard_double_arrow_right Article 01 Jan 2010 English Publisher:WileyJournal:PROTEOMICS, volume 10, pages 337-342 (issn: 1615-9853, eissn: 1615-9861,

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Authors: Srikumar, Tharan; Jeram, Stanley M.; Lam, Henry H N; Raught, Brian;

doi: 10.1002/pmic.200900627

pmid: 19899083

A ubiquitin and ubiquitin‐like protein spectral library

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Abstract

AbstractWe have developed and validated a ubiquitin (Ub) and ubiquitin‐like protein (Ubl) spectral library, consisting of 467 consensus spectra (320 unique peptides derived from autophagy‐related protein 8, F‐adjacent transcript 10, interferon‐stimulated gene 15 kDa protein, neural precursor cell expressed developmentally down‐regulated protein 8, small ubiquitin‐related modifiers 1–3 and Ub, and nine of the most commonly observed Ub/Ubl chain linkages). The use of the Ub/Ubl library with a spectral matching tool (SpectraST) yields improved performance over database search engines, and can successfully identify many types of Ub/Ubl chain‐derived peptides that cannot be identified by standard database search algorithms.

Related Organizations

Ontario Institute for Cancer Research
Canada
Hong Kong Polytechnic University
China (People's Republic of)
Hong Kong University of Science and Technology (香港科技大學)
China (People's Republic of)

Keywords

SpectraST, Ubiquitin, Molecular Sequence Data, MS, Ubiquitin-like protein, Search Engine, Spectral matching, Technology Ubiquitin, Humans, Amino Acid Sequence, Databases, Protein, Sequence Alignment, Ubiquitins, Algorithms

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