Abstract The Speckled Peacock Bass Cichla temensis is a popular sport and food fish with potential for aquaculture. The species is reported to be sensitive to temperature extremes and low dissolved oxygen, but the growth response to these variables has not been evaluated. This study examined temperature (25, 30, and 35°C) effects on growth of juveniles (TL [mean ± SE] = 139 ± 1 mm; wet weight = 29.73 ± 0.64 g) during an 8-week period. Length and weight gains were similar for fish acclimated to 25°C (25 ± 1 mm; 20.03 ± 1.65 g) and 30°C (26 ± 2 mm; 19.17 ± 1.90 g), whereas fish that were held at 35°C had reduced growth (7 ± 1 mm; 0.79 ± 0.55 g). Specific growth rate (SGR; % of wet body weight [BW]/d) and Fulton's condition factor at the end of the experiment (Kf) were similar for fish acclimated to 25°C (0.87 ± 0.05% BW/d; 1.10 ± 0.02) and 30°C (0.85 ± 0.06% BW/d; 1.08 ± 0.02), while those acclimated to 35°C displayed lower SGR and Kf (0.04 ± 0.03% BW/d; 0.97 ± 0.02). Survival was over 90% among all treatments. These results indicate that 25–30°C is preferable to 35°C for rearing juvenile Speckled Peacock Bass and that higher temperatures should be avoided.