AbstractCane molasses contain both protein and non‐protein amino acids and fatty acids. Gas chromatographic evidence is presented for aspartic and glutamic acids and alanine, being the major acids out of the 18 protein amino acids determined. Two unidentified N‐containing free acids, eluting faster than alanine and other protein amino acids on EGA column, are of particular interest. Linoleic (C18:2) is by far the most important fatty acid found. Other major fatty acids are palmitic (C16:0), oleic (C18:1) and arachidic (C20:0).