AbstractClaspers of adult specimens of the skate tribe Riorajini, family Arhynchobatidae, comprising Atlantoraja and Rioraja, are described, compared, and systematically reinterpreted based on material collected off southeastern and southern Brazil. For the first time the external components and musculature of the clasper of members of this tribe are described and related to internal (skeletal) structures. The component pecten is present in all species of Atlantoraja but absent in Rioraja. The new external component grip, an autapomorphy of A. cyclophora fully developed in adults, is described. Rioraja presents dorsal terminals 1 and 2, ventral marginal distally extended and ventral terminal cartilages. Dorsal terminals 1 and 2, ventral marginal distally extended, accessory terminals 2 and 3, and ventral terminal cartilages occur in Atlantoraja. A new interpretation of the ventral marginal distally extended is discussed. The dorsal terminal 1 of Atlantoraja has an inverted U shape but is triangular in Rioraja. The accessory terminal 2 cartilage is reported for the first time in Atlantoraja cyclophora. The accessory terminal 3 is present only in A. platana and A. cyclophora, and absent in Rioraja and A. castelnaui. Many of our findings concerning the clasper skeleton do not agree with previous interpretations. The arrangement, distribution and systematic significance of many of the terminal clasper components are discussed among rajoids.