Although a few measures of disgust propensity are available in Italy, most of them take a long time to administer and/or have not shown replicable and sound psychometric properties. In the current study, the authors developed an Italian nine‐item self‐report measure of disgust propensity (particularly of pathogen disgust)—the Disgust Propensity Questionnaire (DPQ)—to address the limitations of currently available measures. In Study 1, the DPQ was developed through exploratory and confirmatory factor analyses from an initial pool of 33 items that were administered to 784 nonclinical participants. The DPQ showed evidence of an adequate factorial and construct validity as well as internal consistency and temporal stability. In Study 2, additional evidence of the sound psychometric properties of the DPQ was provided by analyzing an independent sample of 315 nonclinical participants and a sample of 208 patients with obsessive–compulsive disorder. This study also showed that the DPQ can discriminate between obsessive–compulsive disorder patients with and without contamination‐related concerns, patients with anxiety disorders, and nonclinical participants.Key Practitioner Message An Italian nine‐item self‐report disgust propensity measure was developed to address the limitations of currently available tools. The Disgust Propensity Questionnaire (DPQ) was evaluated using two independent studies in nonclinical and clinical samples. The DPQ showed adequate factorial and construct validity, internal consistency, and temporal stability. It could discriminate between patients with obsessive–compulsive disorder with contamination‐related concerns and all other groups. It is a very short and psychometrically sound measure to assess disgust propensity in Italian samples.