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Current Protocols in Microbiology
Article . 2017 . Peer-reviewed
License: Wiley Online Library User Agreement
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Epigenetic Analysis of SV40 Minichromosomes

Authors: Balakrishnan, Lata; Milavetz, Barry;

Epigenetic Analysis of SV40 Minichromosomes

Abstract

AbstractSimian virus 40 (SV40) is one of the best‐characterized members of the polyomavirus family of small DNA tumor viruses. It has a small genome of 5243 bp and utilizes cellular proteins for its molecular biology, with the exception of the T‐antigen protein, which is coded by the virus and is involved in regulating transcription and directing replication. Importantly, SV40 exists as chromatin in both the virus particle and intracellular minichromosomes. These facts, combined with high yields of virus and minichromosomes following infection and ease of manipulation, have made SV40 an extremely useful model to study all aspects of eukaryotic molecular biology. This unit describes procedures for working with SV40 and preparing SV40 chromatin from infected cells and virus particles, as well as procedures for using SV40 chromatin to study epigenetic regulation. © 2017 by John Wiley & Sons, Inc.

Country
United States
Keywords

Epigenomics, Polyomavirus Infections, Virus Cultivation, Simian virus 40 (SV40), Simian virus 40, Chromatin immunoprecipitation, Epigenesis, Genetic, Tumor Virus Infections, Minichromosomes, Next-generation sequencing, Animals, Humans, Viral epigenetics

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    popularity
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    Top 10%
    influence
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
8
Top 10%
Average
Average
bronze