AbstractThe Piperaceae family is represented by herbaceous plants, shrubs, and, more rarely, trees. Among the aromatic varieties of this genus, Piper hispidinervum C. DC. stands out. In this work, volatile and non‐volatile extracts from P. hispidinervum were obtained through the application of sequential extraction methods. The results of steam distillation experiments indicated that optimal conditions were achieved when the extractions were conducted at 101 kPa and an initial bed porosity of 0.91. Chemical analysis showed that safrole is the major component present in the essential oil. NMR analysis revealed the presence of 6‐methoxy eugenol for P. hispidinervum extracts obtained by supercritical fluid extraction using water as co‐solvent at 20 MPa and 313.15 K. Mathematical models adjusted well to the experimental data in all conditions investigated. The methodology proposed represents an improvement in the processing of natural products, since the same material is submitted to different technologies, obtaining different compounds with distinct properties.