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Fragmentation of Immunoglobulin G

descriptionPublicationkeyboard_double_arrow_right Article 01 Mar 1997Publisher:WileyJournal:Current Protocols in Immunology, volume 21 (issn: 1934-3671, eissn: 1934-368X,

Authors: Sarah M. Andrew; Julie A. Titus;

doi: 10.1002/0471142735.im0208s21 , 10.1002/0471143030.cb1604s17 , 10.1002/0471142735.im0210as38

pmid: 18432772 , 18228421 , 18432762

Fragmentation of Immunoglobulin G

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Abstract

AbstractThis unit describes procedures for fragmentation of IgG to the monovalent Fab fragment using papain digestion, and to the bivalent F(ab′)2 fragment by pepsin digestion. Alternative methods of fragmentation to F(ab′)2 include use of papain that is preactivated with cysteine and use of the enzyme ficin. These alternate methods are particularly useful for mouse IgG1 antibodies.

Related Organizations

Lancaster University
United Kingdom
National Institute of Health
Pakistan
Chester College of New England
United States
National Cancer Institute
United States

Keywords

T-Lymphocytes, Guinea Pigs, Antigen-Antibody Complex, Immunoglobulin Fab Fragments, Mice, Papain, Humans, Animals, Horses, Complement Activation, Immunoglobulin Fragments, Binding Sites, Goats, Antibody-Dependent Cell Cytotoxicity, Antibodies, Monoclonal, Pepsin A, Immunoglobulin Fc Fragments, Rats, Ficain, Immunoglobulin M, Immunoglobulin G, Cattle, Electrophoresis, Polyacrylamide Gel, Chickens

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