
pmid: 90493
To the Editor.— We enjoyed reading the article by Komaroff et al in the JulyArchives(138:1069-1073, 1978) and agree that the urinalysis, particularly the examination for bacteriuria, can be helpful in discriminating between a urinary tract infection and vaginitis. In their study, Kunin's criterion 1 was used, which considers the counting of one to ten bacteria in an unstained urine sediment in a high-power dry microscopic field as a positive result. With 10 5 /cc bacteria denoting an important level of bacteriuria, Kunin's method results in approximately 12% false-positives and about that many false-negatives. We favor checking for bacteriuria in an unspun urine specimen stained with methylene blue. This technique eliminates variabilities of urine centrifugation and affords better visualization of bacilli; the dye aids in distinguishing cocciform bacteria from urinary debris. 1 Approximately 1/30,000 cc is contained in one highpower field, so that seeing one bacterium per field correlates
Methylene Blue, Bacteriological Techniques, Bacteriuria, Staining and Labeling, Humans, Female, Urine
Methylene Blue, Bacteriological Techniques, Bacteriuria, Staining and Labeling, Humans, Female, Urine
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