
The aim of this work was to evaluate the number of follicles able to ovum pick up (OPU), the quantity, and quality of oocytes from donors showing different blood levels of cholesterol. For this experiment 5 crossbreed cows were used. Before the beginning of the experiment all cows received an intravaginal progesterone releasing device for 8 days. Two days before the device withdraw all cows received 0.150 mg of d-cloprostenol. The cows were submitted to ovum pick up each 4 days in a total of 6 sections. The cows were injected with 0.150 mg of d-cloprostenol each 8 days, at the moment of the device change. After the evaluation of blood cholesterol, cows were divided in two groups: G1 (cholesterol50 mg/dl). There was a difference (p<0.01) between G1 and G2 in the number of follicles/punction, 9.66 and 12.6, respectively, but we didn't observe difference in the number of recovered oocytes and its quality. These results have shown that cows with blood levels of cholesterol higher than 50 mg/dl have a higher number of follicles able to punction.
O objetivo deste trabalho foi avaliar o número de folículos por punção folicular (PF), a quantidade e a qualidade de ovócitos oriundos de vacas com diferentes níveis séricos de colesterol. Para a realização deste experimento foram utilizadas 5 vacas mestiças. Antes do início do experimento, todas as vacas receberam por 8 dias um dispositivo intravaginal liberador de progesterona e dois dias antes da retirada dos dispositivos todas as vacas receberam 0,150 mg de d-cloprostenol. As sessões de PF ocorreram a cada 4 dias, totalizando 6 sessões. Os animais receberam 0,150 mg de d-cloprostenol a cada 8 dias, juntamente com a troca dos dispositivos. Após avaliação do nível sérico de colesterol, as vacas foram divididas em dois grupos: G1 (colesterol50 mg/dl). Foi registrado uma diferença (p0,05) no número de ovócitos coletados e na qualidade dos mesmos. Verificou-se que vacas com níveis de colesterol superior à 50 mg/dl apresentaram um maior número de folículos aptos à punção.
Folliculogenesis, adicionais Qualidade de ovócitos, Oocyte quality, Foliculogênese
Folliculogenesis, adicionais Qualidade de ovócitos, Oocyte quality, Foliculogênese
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