Spektroskopijom površinski pojačanog Ramanova raspršenja (SERS) istraţivane su interakcije N,N,N-tri(8-piren-1-il-[3,7-diazaokt-1-il])amina (spoj 1) s polinukleotidima. Kao metalni supstrati korišteni su svjeţe pripravljeni, koncentrirani i agregirani koloid. Spoj 1 najintenzivnije raspršuje zračenje pri koncentraciji 5×10−6 mol dm−3 u koncentriranom koloidu te je navedeni supstrat korišten pri pripravi smjesa s polinukleotidima. Smjese su pripremane na dva načina; kod prvog načina u koloid je dodan istraţivani spoj, a zatim odabrani polinukleotid, dok se kod drugog načina prvo pripremio kompleks spoja 1 i polinukleotida, koji je zatim dodan u koloidnu suspenziju. Dobiveni SERS spektri svih smjesa nisu sadrţavali nove vrpce koje bi odgovarale vibracijama polinukleotida. Adsorpcija molekula spoja 1 na nanočestice je prilično jaka i dodatak polinukleotida slabo utječe na intenzitet i poloţaj vrpca. Kompleks spoj 1/polinukleotid daje slabe vrpce poliaminskog derivata čak i pri velikom suvišku polinukleotida, vrlo vjerojatno zbog interkaliranja pirenskog sustava izmeĎu parova baza DNA analoga, dok nevezani dio molekule spoja 1 ostaje blizu metalne površine.

Surface-enhanced Raman scattering (SERS) was used for studying interactions between pyrene derivative of polyamine (1) and polynucleotides. Freshly prepared, concentrated and aggregated colloids were used as metal substrates. Compound 1 scattered radiation most intensively at concentration of 5 × 10−6 mol dm−3 in concentrated colloid, which was therefore used in the preparation of mixtures with polynucleotides. The mixtures were prepared in two ways; firstly, 1 was added to the colloid, followed by adding selected polynucleotide, while in the second method 1/polynucleotide complex was prepared, and then mixed with the colloid. The obtained SERS spectra of all mixtures showed lack of polynucleotides bands. The polyamine adsorption on the silver was quite strong and the addition of polynucleotides poorly effected the bands intensity and position. The compound 1/polynucleotide complex yielded weak bands originating from compound 1, even with a large excess of polynucleotides, most likely due to intercalation of pyrene part between base pairs of DNA analoges, while unbonded part of polyamine molecules remain close to metal surface.