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Роль щитка в прорастании зерновок пшеницы

Роль щитка в прорастании зерновок пшеницы

Abstract

Scutellum functions of wheat kernels (WK) are diverse and caused by the action antioxidant system which is capable to suppress free radicals generation including active forms of oxygen, and to control lipid peroxidation (LPO). The research goal was studying scutellum role in WK germination. The following objectives were involved: 1) studying the state of scutellum antioxidant systems at swelling; 2) defining LPO level and antioxidants content in germ, scutellum and endosperm at the initial stage of water entry in WK; 3) defining enzymes activity during swelling in various parts of WK; and 4) substantiating scutellum role in WK germination. The studied wheat kernels (Triticum aestivum L.) of Prilenskaya 19 variety were soaked in distilled water for 24 h. Kernel number per one dish was 100. The studies were conducted in three biological replications (3-4 analytic replications in each). Test samples were taken at the same time of a day. The initial stages of WK germination were studied. The activity of antioxidant system components and enzymes in WK germ, scutellum and endosperm was defined. The interrelation and dependence between LPO level and antioxidants content in various parts of WK at their swelling was revealed. Water entry activates metabolic processes in various parts of kernels. LPO level in germ and endosperm correlates with the antioxidants content in those parts of kernels, while in scutellum, inverse correlation of those indices is observed. The dynamics of enzymes activity reveals both minima and maxima.

Исследованы начальные этапы прорастания зерновок пшеницы. Определена активность компонентов антиоксидантной системы и ферментов в зародыше, щитке и эндосперме зерновок пшеницы. Установлены взаимная связь и зависимость между уровнем ПОЛ и содержанием антиоксидантов в различных частях зерновок пшеницы в период их набухания. Показано, что поступление воды активизирует активность метаболических процессов в различных частях зерновок. При этом уровень ПОЛ в зародыше и эндосперме коррелятивно связан с содержанием антиоксидантов в этих частях зерновок, тогда как в щитке отмечается обратная корреляции этих параметров. В динамике активностей ферментов (алкоголь-, глюкозо-6-фосфатдегидрогеназы и пероксидаза) отмечаются как минимумы, так и максимумы проявления активности. Поэтому в процессе набухания наблюдается импульсивный характер проявления активности исследованных ферментов, обусловливающий их индивидуальную ритмику. Низкая пероксидазная активность и снижение содержания антиоксидантов в щитке в период набухания свидетельствуют о том, что в этой части зародыша создаются условия для протекания свободнорадикальных реакций.

Keywords

ФИЗИОЛОГИЯ РАСТЕНИЙ, ПОКОЙ, ЗЕРНА ПШЕНИЦЫ, ПРОРАСТАНИЕ, ЭНДОСПЕРМ, ЗАРОДЫШ, ЩИТОК, АНТИОКСИДАНТЫ, ПЕРОКСИДАЗА, АЛКОГОЛЬДЕГИДРОГЕНАЗА, ГЛЮКОЗО-6-ФОСФАТДЕГИДРОГЕНАЗА

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
0
Average
Average
Average
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