Identification of a tRNA-Specific Nuclear Export Receptor
Copyright policy )Identification of a tRNA-Specific Nuclear Export Receptor
In eukaryotes, tRNAs are synthesized in the nucleus and after several maturation steps exported to the cytoplasm. Here, we identify exportin-t as a specific mediator of tRNA export. It is a RanGTP-binding, importin beta-related factor with predominantly nuclear localization. It shuttles rapidly between nucleus and cytoplasm and interacts with nuclear pore complexes. Exportin-t binds tRNA directly and with high affinity. Its cellular concentration in Xenopus oocytes was found to be rate-limiting for export of all tRNAs tested, as judged by microinjection experiments. RanGTP regulates the substrate-exportin-t interaction such that tRNA can be preferentially bound in the nucleus and released in the cytoplasm.
- Max Planck Society Germany
- University of Geneva Switzerland
- Heidelberg University Germany
- German Cancer Research Center Germany
570, Cytoplasm, Nucleocytoplasmic Transport Proteins, RNA, Transfer, Leu, Xenopus, Molecular Sequence Data, Ran GTP-Binding Protein, Beta Karyopherins, Nuclear Proteins / genetics, Xenopus Proteins, RNA, Transfer, GTP-Binding Proteins, Cytoplasm / metabolism, Cell Nucleus / chemistry, RNA, Messenger / analysis, Animals, Humans, Amino Acid Sequence, RNA, Messenger, GTP-Binding Proteins / metabolism, Cloning, Molecular, Molecular Biology, RNA, Transfer, Ser, Cell Nucleus, Cell Nucleus / metabolism, Nuclear Proteins / metabolism, Protein Binding / physiology, GTPase-Activating Proteins, Carrier Proteins / metabolism, RNA, Transfer, Amino Acid-Specific / metabolism, Nuclear Proteins, Cell Biology, RNA, Transfer, Ser / metabolism, RNA, Transfer, Amino Acid-Specific, RNA, Transfer, Leu / metabolism, Oocytes, RNA, Transfer / metabolism, Carrier Proteins, Carrier Proteins / genetics, Oocytes / physiology, HeLa Cells, Protein Binding
570, Cytoplasm, Nucleocytoplasmic Transport Proteins, RNA, Transfer, Leu, Xenopus, Molecular Sequence Data, Ran GTP-Binding Protein, Beta Karyopherins, Nuclear Proteins / genetics, Xenopus Proteins, RNA, Transfer, GTP-Binding Proteins, Cytoplasm / metabolism, Cell Nucleus / chemistry, RNA, Messenger / analysis, Animals, Humans, Amino Acid Sequence, RNA, Messenger, GTP-Binding Proteins / metabolism, Cloning, Molecular, Molecular Biology, RNA, Transfer, Ser, Cell Nucleus, Cell Nucleus / metabolism, Nuclear Proteins / metabolism, Protein Binding / physiology, GTPase-Activating Proteins, Carrier Proteins / metabolism, RNA, Transfer, Amino Acid-Specific / metabolism, Nuclear Proteins, Cell Biology, RNA, Transfer, Ser / metabolism, RNA, Transfer, Amino Acid-Specific, RNA, Transfer, Leu / metabolism, Oocytes, RNA, Transfer / metabolism, Carrier Proteins, Carrier Proteins / genetics, Oocytes / physiology, HeLa Cells, Protein Binding
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